PFLISA-Flex
A robust, versatile platform for developing homebrew, ultrasensitive
singleplex immunoassays. The kit enables rapid customization & seamless transition
from existing platforms
● 50-1000X higher sensitivity than ELISA with extended dynamic range
● Fast, Easy, Ultrasensitive Assay Development
● Familiar ELISA-like workflow
Ultrasensitive Singleplex Homebrew Assay
A robust and versatile platform that supports rapid customization & deployment of tailored PFLISA
assays with use of your own antibodies and reagents
No Capture Antibody Labeling Required
50-1000X higher sensitivity than ELISA
Between 4 and 5 orders of magnitude of logarithmic dynamic range
No color development & signal stable for 24 hours
Less than 13 minutes read times and software completes analysis
Undiluted sample volume less than 15μL
Ultrahigh Sensitivity
>50X higher sensitivity than ELISA
Consistent Reproducibility
| Human IL-6 | Intra-plate %CV (n=8) | Interplate %CV (n=2) | ||
|---|---|---|---|---|
| Spike (pg/mL) | ELISA | PFLISA-FLEX | ELISA | PFLISA-FLEX |
| 300 | 4.9% | 8.5% | 4.7% | 4.2% |
| 18.2 | 8.2% | 8.5% | 0.3% | 2.7% |
| 1.2 | 43.7% | 9.2% | 17.8% | 10.6% |
| Human IL-1b | Intra-plate %CV (n=8) | Interplate %CV (n=2) | ||
|---|---|---|---|---|
| Spike (pg/mL) | ELISA | PFLISA-FLEX | ELISA | PFLISA-FLEX |
| 300 | 10.6% | 7.5% | 1.7% | 1.1% |
| 18.2 | 10.9% | 7.4% | 4.0% | 4.9% |
| 1.2 | 32.4% | 6.0% | 13.3% | 2.3% |
Easy, Familiar, Assay Workflow
Versatile Assay Platform
