Ultrasensitive.
No Compromise.

One immunoassay platform with breakthrough sensitivity, multiplex capability, and broad dynamic range — without giving up the ELISA workflow your lab already runs.

fg/mL true LLOQ
1 → N single to multiplex, same result
1 platform discovery to clinical
Brightest Bio Product


What Does "No Compromise" Actually Mean?

Scientists are used to making compromises.

ELISA

Familiar workflow, but low sensitivity, small dynamic range & large sample volume

High-sensitivity platforms

Better sensitivity, poor multiplexing, but low flexibility, complicated instrumentation and higher costs

Immuno-PCR systems

More analytes, but reduced assay performance and reliability

Multiple different platforms

Potentially viable, but requires additional development, validation, time and expense


Why Scientists Shouldn't Have to Choose

The Four No-Compromise Advantages

Sensitivity

No Compromise on Sensitivity

Detect what others miss.

PFLISA delivers 50–1000x higher sensitivity leading to consistent femtogram-level LLOQs with low picogram single digit CV while preserving a familiar ELISA workflow.

Workflow

No Compromise on Workflow

Keep the process. Improve the performance.

Move from multiplex screening to focus-plex or singleplex confirmation with validated multiplex panels without changing platforms, methods, instruments, or critical reagents.

Flexibility

No Compromise on Flexibility

One platform that adapts to your science.

Choose ready-to-run multiplex panels, create homebrew singleplex assays with your own reagents, or configure your own focus-plex panels from our validated antibody pairs.

Dynamic Range

No Compromise on Dynamic Range

Measure a wider range with one dilution.

PFLISA provides between 4 and 5 orders of magnitude of logarithmic dynamic range enabling studying a wider range of sample concentrations with one dilution on one plate.


Ultrasensitivity should not require a high-sensitivity budget

Breakthrough Performance

Lower Capital Investment

Reduced Operating Cost


Most Platforms Make You Sacrifice Something

Brightest Bio Assay Comparison
× Brightest Bio Assay Comparison

PFLISA eliminates these trade-offs.


The Product Ecosystem

One Platform. Three Formats. Endless Possibilities.


PFLISA™ supports the entire assay lifecycle on a single platform. No redevelopment. No revalidation. No compromise.

Contact Us
Ultrahigh Performance

No Compromise
on Sensitivity

50–1000x signal amplification with femtogram-level detection

Affordable

No Compromise
on Workflow

Maintain the ELISA workflow your team already uses

Fast and Simple

No Compromise
on Flexibility

Multiplex, Singleplex and Focused-Plex on one platform

Higher Productivity

No Compromise
on Dynamic Range

Measure a wider range of sample concentrations with one dilution

Higher Productivity

No Compromise
on Cost

High-performance immunoassays at lower capital cost


Why scientists are moving to PFLISA

  • 50x - 1000x lower LLOQ than traditional ELISA
  • Consistent LLOQ for singleplex to multiplex
  • Direct ELISA transfer with no optimization
  • Single-digit CVs
  • Robust signal stability
  • Rapid in-house assay development
  • Automation-friendly workflows
  • Delivering ultrasensitive immunoassays without compromise


ELISA Has Evolved to PFLISA™

No compromise on Sensitivity. Workflow. Flexibility. Cost.

Slide 1
Slide 2
Slide 3


Get the performance today’s research demands without changing how your lab works.



Contact Us
to find out how the PFLISA™ platform can revolutionise your immunoassay analysis